No.
All existing Real-Time migration settings stay intact after the upgrade.
However, new options (like parent record filtering) can be configured anytime by the admin. 
PS: View Drag, Drop & Upload Files to Amazon S3, a Salesforce AppExchange app for moving files from Salesforce to Amazon S3: https://appexchange.salesforce.com/appxListingDetail?listingId=a0N3000000B5ZPqEAN